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This article provides an in-depth analysis of the TypeError: must be str, not bytes error encountered when handling subprocess output in Python 3. By comparing the string handling mechanisms between Python 2 and Python 3, it explains the fundamental differences between bytes and str types and their implications in the subprocess module. Two main solutions are presented: using the decode() method to convert bytes to str, or directly writing raw bytes via sys.stdout.buffer.write(). Key details such as encoding issues and empty byte string comparisons are discussed to help developers comprehensively understand and resolve such compatibility problems.