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This article provides a comprehensive guide to displaying external image files in Jupyter Notebook, with detailed analysis of the Image class in the IPython.display module. By comparing implementation solutions across different scenarios, including single image display, batch processing in loops, and integration with other image generation libraries, it offers complete code examples and best practice recommendations. The article also explores collaborative workflows between image saving and display, assisting readers in efficiently utilizing image display functions in contexts such as bioinformatics and data visualization.