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This technical paper comprehensively examines string processing challenges encountered when handling NCBI reference sequence accession numbers in the R programming environment. Through detailed analysis of real-world scenarios involving version suffix removal, the article elucidates the critical importance of special character escaping in regular expressions, compares the differences between sub() and gsub() functions, and provides complete programming solutions. Additional string processing techniques from related contexts are integrated to demonstrate various approaches to string splitting and recombination, offering practical programming references for bioinformatics data processing.